| 质粒名称: | pTRIP-SFFV-mtagBFP-2A STING |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 102586 |
| 存储实验室: | Nicolas Manel |
|
载体骨架基本信息 |
|
| 载体名称: | pTRIP |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | STING |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | SFFV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | SFFV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTRIP-SFFV-mtagBFP-2A STING was a gift from Nicolas Manel (Addgene plasmid # 102586 ; http://n2t.net/addgene:102586 ; RRID:Addgene_102586) |
| 参考文献部分: | Intrinsic antiproliferative activity of the innate sensor STING in T lymphocytes. Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel N. J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. Epub 2017 May 8. 10.1084/jem.20161674 PubMed 28484079 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTRIP-SFFV-mtagBFP-2A STING 11496 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Lentivector to express STING and mTagBFP.
ACCESSION .
VERSION .
KEYWORDS pTRIP-SFFV-mtagBFP-2A STING
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11496)
AUTHORS Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg
MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
N
TITLE Intrinsic antiproliferative activity of the innate sensor STING in T
lymphocytes.
JOURNAL J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674.
Epub 2017 May 8.
PUBMED 28484079
REFERENCE 2 (bases 1 to 11496)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11496
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 43..108
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 238..258
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 683..817
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(720..739)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 774..793
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 985..1618
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 1665..1790
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 2283..2516
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 3038..3155
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3295..3702
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 3721..4419
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
DLPSKLGHKLN"
CDS 4429..4485
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
LTR 5900..6080
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7608..7627)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(7841..7860)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7960..7982
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(8020..8038)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8106..8210
/gene="bla"
/label=AmpR promoter
CDS 8211..9071
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8429..8448)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9242..9830
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9731..9750
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9984..10001
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(10071..10091)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10076..10405
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10256..10391
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10318..10337
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
ORIGIN
1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc catgccccac
4501 tccagcctgc atccatccat cccgtgtccc aggggtcacg gggcccagaa ggcagccttg
4561 gttctgctga gtgcctgcct ggtgaccctt tgggggctag gagagccacc agagcacact
4621 ctccggtacc tggtcctcca cctagcctcc ctgcagctgg gactgctgtt aaacggggtc
4681 tgcagcctgg ctgaggagct gcgccacatc cactccaggt accggggcag ctactggagg
4741 actgtgcggg cctgcctggg ctgccccctc cgccgtgggg ccctgttgct gctgtccatc
4801 tatttctact actccctccc aaatgcggtc ggcccgccct tcacttggat gcttgccctc
4861 ctgggcctct cgcaggcact gaacatcctc ctgggcctca agggcctggc cccagctgag
4921 atctctgcag tgtgtgaaaa agggaatttc aacgtggccc atgggctggc atggtcatat
4981 tacatcggat atctgcggct gatcctgcca gagctccagg cccggattcg aacttacaat
5041 cagcattaca acaacctgct acggggtgca gtgagccagc ggctgtatat tctcctccca
5101 ttggactgtg gggtgcctga taacctgagt atggctgacc ccaacattcg cttcctggat
5161 aaactgcccc agcagaccgg tgaccgtgct ggcatcaagg atcgggttta cagcaacagc
5221 atctatgagc ttctggagaa cgggcagcgg gcgggcacct gtgtcctgga gtacgccacc
5281 cccttgcaga ctttgtttgc catgtcacaa tacagtcaag ctggctttag ccgggaggat
5341 aggcttgagc aggccaaact cttctgccgg acacttgagg acatcctggc agatgcccct
5401 gagtctcaga acaactgccg cctcattgcc taccaggaac ctgcagatga cagcagcttc
5461 tcgctgtccc aggaggttct ccggcacctg cggcaggagg aaaaggaaga ggttactgtg
5521 ggcagcttga agacctcagc ggtgcccagt acctccacga tgtcccaaga gcctgagctc
5581 ctcatcagtg gaatggaaaa gcccctccct ctccgcacgg atttctcttg actcgagacc
5641 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
5701 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
5761 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
5821 tggaagggct aattcactcc caacgaagac aaaatcgtcg agagatgctg catataagca
5881 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
5941 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
6001 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
6061 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
6121 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa
6181 ttgaactagg agtggagcac acaggcaaag ctgcagaagt acttggaaga agccaccaga
6241 gatactcacg attctgcaca tacctggcta atcccagatc ctaaggatta cattaagttt
6301 actaacattt atataatgat ttatagttta aagtataaac ttatctaatt tactattctg
6361 acagatatta attaatcctc aaatatcata agagatgatt actattatcc ccatttaaca
6421 caagaggaaa ctgagaggga aagatgttga agtaattttc ccacaattac agcatccgtt
6481 agttacgact ctatgatctt ctgacacaaa ttccatttac tcctcaccct atgactcagt
6541 cgaatatatc aaagttatgg acattatgct aagtaacaaa ttaccctttt atatagtaaa
6601 tactgagtag attgagagaa gaaattgttt gcaaacctga atagcttcaa gaagaagaga
6661 agtgaggata agaataacag ttgtcattta acaagtttta acaagtaact tggttagaaa
6721 gggattcaaa tgcataaagc aagggataaa tttttctggc aacaagacta tacaatataa
6781 ccttaaatat gacttcaaat aattgttgga acttgataaa actaattaaa tattattgaa
6841 gattatcaat attataaatg taatttactt ttaaaaaggg aacatagaaa tgtgtatcat
6901 tagagtagaa aacaatcctt attatcacaa tttgtcaaaa caagtttgtt attaacacaa
6961 gtagaatact gcattcaatt aagttgactg cagattttgt gttttgttaa aattagaaag
7021 agataacaac aatttgaatt attgaaagta acatgtaaat agttctacat acgttctttt
7081 gacatcttgt tcaatcattg atcgaagttc tttatcttgg aagaatttgt tccaaagact
7141 ctgaaataag gaaaacaatc tattatatag tctcacacct ttgttttact tttagtgatt
7201 tcaatttaat aatgtaaatg gttaaaattt attcttctct gagatcattt cacattgcag
7261 atagaaaacc tgagactggg gtaattttta ttaaaatcta atttaatctc agaaacacat
7321 ctttattcta acatcaattt ttccagtttg atattatcat ataaagtcag ccttcctcat
7381 ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca aaaatattgg gaaaaaatta
7441 aaaatagcaa tacaacaata aaaaaataca aatcagaaaa acagcacagt ataacaactt
7501 tatttagcat ttacaatcta ttaggtatta taagtaatct agccagatcc tctacgccgg
7561 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
7621 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
7681 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
7741 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
7801 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
7861 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
7921 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
7981 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
8041 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
8101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
8161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
8221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
8281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
8341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
8401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
8461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
8521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
8581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
8641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
8701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
8761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
8821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
8881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
8941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
9001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
9061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
9121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
9181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
9241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
9301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
9361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
9421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
9481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
9541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
9601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
9661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
9721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
9781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
9841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
9901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
9961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
10021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
10081 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
10141 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
10201 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
10261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
10321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
10381 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
10441 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
10501 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
10561 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
10621 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
10681 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
10741 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
10801 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
10861 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
10921 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
10981 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
11041 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
11101 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
11161 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
11221 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
11281 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
11341 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
11401 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
11461 cggatctttg tgaaggaacc ttacttctgt ggtgtg
//
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